Irf3 mouse

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August undefined, 2024

WebOct 15, 2013 · Double-deficient Irf-3(-/-)7(-/-) mice infected with the DENV2 strain S221 possessed 1,000-150,000 fold higher levels of viral RNA than wild-type and single-deficient mice 24 h postinfection (hpi); however, they remained resistant to lethal infection.

IRF3 - Wikipedia

IRF3 is a member of the interferon regulatory transcription factor (IRF) family. IRF3 was originally discovered as a homolog of IRF1 and IRF2. IRF3 has been further characterized and shown to contain several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain and several regulatory phosphorylation sites. IRF3 is found … WebThe IRF3 conditional knockout mouse may be a useful tool to identify cell type-specific functions of this transcription factor. Abstract IFN regulatory factor 3 (IRF3) is a … small 4th of july clipart

IRF3 inhibits nuclear translocation of NF-κB to prevent viral ...

WebE. coli -derived recombinant mouse IRF3 Thr201-Ile419 Accession # P70671 Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is … WebApr 13, 2024 · Knockdown of STING (stimulator of interferon genes), TBK1 (TANK-binding kinase-1), or IRF3 (interferon regulatory factor-3) also resulted in increased micronuclei formation. WebIrf3 Organism names Organism Mus musculus (Mouse) Taxonomic identifier 10090 NCBI Taxonomic lineage Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > … small 4wh drive truck rentals

Recombinant Anti-IRF3 antibody [EPR2418Y] (ab68481) - Abcam

54131 - Gene ResultIrf3 interferon regulatory factor 3 [ (house mouse)]

WebJan 18, 2024 · The TFs interferon regulatory factor 3 (IRF3), IRF5 and IRF7 (IRF3/5/7) ... To understand the role of DNA binding in IFN regulation, we mapped the binding of IRF3/5/7 dimers to all human and mouse IFN gene VREs. We found that IRF3 and IRF7 bind across many of the VRE sub-elements, but that the VRE-A/B sub-element is bound almost … WebIrf3 MGI Mouse Gene Detail - MGI:1859179 - interferon regulatory factor 3 Search Submit Data Find Mice (IMSR) Analysis Tools Contact Us Browsers Irf3 Gene Detail Summary … solid edge pcb collaborationWebMar 5, 2024 · (C) Immunoblot analysis of p-TBK1, p-IRF3, and IRF3 in mouse PMs transfected with Ctrl siRNA or Rnf39 siRNA 2 for 48 hours and then infected with VSV for the indicated time periods. ( D ) Luciferase activity analysis of IRF3 promoter activity in HEK293T cells transfected with IRF3 reporter plasmid and the indicated adaptor … small 4th of july decorations

"WebIRF3 Antibodies. Antibodies that detect IRF3 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry … " - Irf3 mouse

Irf3 mouse

Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb - Cell Signaling …

WebUsing cellular and mouse models, we reveal IRF3, a critical component of innate antiviral immunity, inhibits the activity of NF-κB, the pro-inflammatory transcription factor. … WebMar 26, 2015 · Interferon Regulatory Factor 3 (IRF3) is an essential transcription factor for the expression of antiviral genes, including type I IFNs and ISGs. ... Ser398 in human IRF3 …

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WebWestern blot analysis of extracts of NIH/3T3 cells, using Phospho-IRF3-S396 antibody (AP0623) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. WebIn this study, we analyzed the role of IRF3 and IRF7 for the host response to IAV infections in Irf3-/-, Irf7-/-, and Irf3-/-Irf7-/- knockout mice. While the absence of IRF3 had only a …

WebImmunogen Synthetic peptide within Human IRF3 aa 50-150. The exact sequence is proprietary. Database link: Q14653 (Peptide available as ab203561) Positive control WB: A549, HeLa, Jurkat, THP-1, Daudi, HepG2 whole cell lysates. Human fetal heart and kidney lysates. Mouse heart and spleen lysates, NIH/3T3 whole cell lysates. WebAnti-IRF3 antibody (ab46194) was used in the publication: Boxer EL et al. The rinderpest virus non-structural C protein blocks the induction of type 1 interferon. Virology 385:134 …

http://www.informatics.jax.org/marker/MGI:1859179 WebIn select wells, rabbit anti-phospho IRF3 (S386) antibody is added to detect phosphorylated IRF3. In the remaining wells, mouse anti-pan-IRF3 antibody is used to detect pan IRF3. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted into the wells.

WebWe previously showed that interferon regulatory factor 3 (IRF3) contributes to sepsis pathogenesis in young mice subject to cecal ligation and puncture (CLP). In this study, we investigated if IRF3 contributes to sepsis in the context of aging. ... Two-way repeated measures ANOVA was used to compare the mouse surface temperature and disease ...

WebFor our assay, we are attempting to detect IRF3 activation via dimerization in mouse macrophage nuclear and cytoplasmic lysates run on a native gel. It is important for us … small 4 wheel drive puWebFurthermore, pBud-H5-GFP co-expressing IRF3 as a genetic adjuvant demonstrated the highest hemagglutination inhibition titer besides larger CD3+/CD4+ and CD3+/CD8+ T cells population, and strong Th1-like cytokine responses among all the systems tested. ... In this study, mouse cytokines, including pro-inflammatory cytokines, (IL-1β, TNF-α ... small 4 tier greenhouseWebBuy Irf3, Mouse interferon regulatory factor 3, Real Time PCR Primer Set, item number: VMPS-3157 from RealTimePrimers at Biomol! Primers are provided as a 40 µl solution … small 4wd suv reviewsWebApr 11, 2024 · For mouse studies with IFNAR antibody treatment, following streptomycin treatment as described above, mice were injected intraperitoneally with 250 µg of isotype control (In vivo Mouse IgG1 ... solid edge rectangular patternWebWe uncovered a general mechanism of IRF3 activation by the innate immune adaptor proteins MAVS, STING, and TRIF, which functions in three distinct pattern recognition pathways. Following its activation, each adaptor protein recruits and activates downstream kinase TBK1, which phosphorylates the cognate upstream adaptor protein at a consensus … solid edge rotate assembly partWebNov 1, 2012 · IFN regulatory factor 3 (IRF3) regulates early type I IFNs and other genes involved in innate immunity. We have previously shown that cells undergoing an endoplasmic reticulum (ER) stress response called the unfolded protein response produce synergistically augmented IFN-β when stimulated with pattern recognition receptor … solid edge schrauben downloadWebNov 2, 2024 · To identify potential IRF3-associated lncRNAs in vivo, we first generated the genetic manipulated mice with a FLAG-tag sequence inserted at the 3′ end of the coding sequence of Irf3 genes (Irf3 flag/flag) to facilitate the purification of endogenous IRF3 protein.Then we performed FA-CLIP with anti-FLAG antibody in primary mouse peritoneal … solid edge rotate text profile