Pac5.1/v5-his c

Author: diqr

August undefined, 2024

WebAlias:pAc5.1/V5-HisCPlasmid type:Drosophila cell expression vectorPromoter:pAC5Cloning Method:Multiple cloning sites,restriction endonucleaseSize:5371 bp 5' Sequencing … Web丁香通商铺武汉华尔纳生物科技有限公司为您提供细胞库 / 细胞培养、elisa 试剂盒、抗体、试剂、耗材等分类产品11件，全方位满足您的科研实验采购需求

Addgene: Vector Database - pAc5.1/V5-His C

WebThe DES®-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin … WebGH3, a novel proapoptotic domain in Drosophila Grim, promotes a mitochondrial death pathway fchd ironbridge

Generation of stable Drosophila cell lines using multicistronic …

WebItem pAc5.1/V5-His A, B, & C; Company Thermo Fisher Scientific; Catalog Number V411020; This product is no longer available on Biocompare. Biocompare is the leading resource for … WebpAc5.1 V5-His A Vector for constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells. For other reading frames, use pAc5.1/V5-His B or pAc5.1/V5-His C. … WebApr 12, 2024 · Ekaete - S01 E05. 23 mins ago. Read Story: SEASON 1 EPISODE 5. I felt myself getting closer to my c—-x. His lips went to my n!pples and bit lightly on the left while he had the other between his fingers. I bucked my hips into his and I felt him moan into my boobs. Me: Ohhh sit.. this feels so good. I confessed. I felt myself get closer to my ... fchd.info chelsea

pAc5.1/V5-His A, B, C 5.4 kb - HonorGene

WebBriefly, the purified cloned vector (pAc5.1/V5-His/Kaz-S) was transfected by use of calcium phosphate (Invitrogen) into 2 × 10 6 to 4 × 10 6 S2 cells/ml in six-well cell culture plates (Costar). The cells were harvested at 48, 72, 96, 120, and 144 h after plasmid transfection. http://www.labbase.net/Supply/SupplyItems-4229347.html fchd herefordWeb常见载体引物表 Vector PBAD pACT pAc5.1/V5-His Primer(F) PBAD-F/TRXF T7 Ac5-Profor GAL4-AD-Cfor Primer(R) ... 克隆载体 M13KO7 Helper Phage Plasmid pBR322 pUni/V5-His A, B, C ... fchd gateshead

"WebAug 31, 2011 · Here, we describe pAc5-STABLEs, new vectors for use in Drosophila cell culture to facilitate stable transformation. We show that viral-derived 2A-like (or "CHYSEL") peptides function in... " - Pac5.1/v5-his c

Pac5.1/v5-his c

Invitrogen pAc5.1/V5-His A, B, C Vectors 20μg:Molecular …

WebpAc5.1/V5-His A载体/pAc5.1/V5-His A质粒图谱/pAc5.1/V5-His A序列已经提出过许多方法用于从细菌中提纯质粒DNA，这些方法都含有以下3个 ... WebpAc5.1/V5-His A, B, or C in frame with the C-terminal peptide encoding the V5 epitope and the polyhistidine tag (if desired). “Cloning considerations” on page 8–“Multiple cloning site …

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WebAmpicillin resistance (bla) gene (ORF): bases 4316-5176 (c) The sequences of pAc5.1/V5-His A, B, and C have been compiled from information in sequence databases, published … WebMay 8, 2003 · C. pipiens ace-1 encodes a putative 702-amino-acid protein, ... S2 cells were transfected with the recombinant pAc5.1/V5-His vector (Invitrogen) either alone (purple circles) or with expression of ...

WebThe pAC5.1/V5-His vectors can be used with the DES™-Inducible Kits (K512001 and K412001) for constitutive expression of your protein of interest. The pAc5.1/V5-His vectors also offer the following features: • Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency WebBy using the pMT/BiP/V5-His vector, you’ll get secretion of native protein for improved yield and protein quality. Fast cloning saves time TOPO® Cloning is the fastest method for …

WebRCASBP-AP (A) (CC#37) 价格： 1200元. 联系方式： I47-825O-882O. 相关技术服务：质粒构建基因合成质粒大提. 立刻购买. WebApr 22, 2024 · The PCR-amplified H2B-mCherry-HA was then inserted into the XhoI site of the Drosophila constitutive expression vector, pAc5.1/V5-His version C (V411020, Thermo Fisher Scientific). In order to test the transferring ability of gypsy, the gypsy backbone used in previous publication [ 11 ] was amplified and cloned into the NotI/XbaI sites of pAc5 ...

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WebpAc5.1 V5-His B. Vector for constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells. For other reading frames, use pAc5.1/V5-His A or pAc5.1/V5-His … fchd new miltonWebFeb 26, 2007 · The coding sequence for the signal sequence of premembrane and E protein was cloned into the Drosophila expression vector pAc5.1/V5-His. A Drosophila cell line S2 was cotransfected with this construct as well as a … fchd hereford unitedWebThe pAC5.1/V5-His vectors can be used with the DES™-Inducible Kits (K512001 and K412001) for constitutive expression of your protein of interest. The pAc5.1/V5-His vectors also offer the following features: • Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency fchd leatherhead