Phospho erk assay
WebDec 2, 2016 · Of our 24 samples, 4 samples were excluded from analysis due to poor viability (<40% live cells), giving an assay success rate of 83.3%. We were able to successfully measure p-ERK in all 20 viable patient samples and also measure levels of baseline p-AKT in 16 samples. WebNov 15, 2014 · Extracellular signal-regulated kinase (ERK) controls various aspects of cell physiology including autophagy. It has been shown that growth factor increases the interaction of ERK cascade components with autophagy (ATG) proteins in …
Phospho erk assay
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WebJun 25, 2015 · Phospho-ERK Assays - Assay Guidance Manual - NCBI Bookshelf … WebSep 1, 2016 · This technique termed phospho-flow cytometry has shown significant promise in stratification of disease ( Irish et al., 2004, Brown et al., 2015 ), monitoring disease progression ( Cesano et al., 2013) and identification of biomarkers for monitoring therapeutic intervention ( Gavasso et al., 2014 ).
WebJun 23, 2014 · We report the development of a new cell-based Phospho-ERK1/2 assay (designated Phospho-ERK), which is a sandwich proximity-based assay using the homogeneous time-resolved fluorescence technology. WebAs shown in Fig. 4 A , both iso- forms of ERK were equally phosphorylated. These effects were further quantified using an ELISA-based assay to compare the levels of ERK phosphorylation in...
Web48-618MAG Millipore MILLIPLEX® Total/Phospho Akt - Cell Signaling Multiplex Assay Download Zoom Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate sa More>> WebAssay IDs Entrez Gene IDs Gene Symbols Gene Aliases COSMIC IDs SNP IDs Nucleotide Mutations (e.g. c.2582T>A) Amino Acid Changes (e.g. p.L861Q) RefSeq Accessions. Supported Keywords: Gene symbol Assay ID Plate SKU. Supported Keywords: miRBase ID Assay ID Plate SKU. Supported Keywords: Gene Name Gene ID Assay ID. Supported …
WebOct 15, 2004 · The assay uses infrared-labeled secondary antibodies to detect phospho-ERK, and the signals in the wells of the microtiter plate are quantified by a LI-COR infrared scanner. V (max), EC (50), and functional K (i) values of various D2 and D3 agonists and antagonists determined in this assay are similar to those in the literature.
WebERK: An extracellular signal-regulated kinase family, also known as a mitogen activated protein kinase (MAPK), consisting of ERK1 and ERK2 that share ~85% sequence identity An overview of the RAS-RAF-MEK-ERK pathway. Contact us to learn more about our RAS pathway profiling and assay development services. Target Engagement cye22tshss filterWebsignals from each assay well to easily normalize the assay. Here, we demonstrate the benefit and utility of normalizing assay signal of phosphorylated protein levels to total protein levels in two different cellular models: ERK 1/2 phosphorylation in human melanoma A375 cells and AKT 1/2/3 phosphorylation in mouse myoblast C2C12 cells. cye22tshss geWebMay 26, 2014 · The objective of this study is to investigate the contributing effect of the nuclear transcription factor-erythroid 2-related factor 2 (Nrf2)-mediated signaling pathway on the indirect antioxidant capacity of caffeic acid phenethyl ester (CAPE) against oxidative stress in HepG2 cells. The result of an antioxidant response element (ARE)-luciferase … cye22tp2mds1WebERK phosphorylation was detectable as early as 1 min after agonist addition, with a … cye22tshss ge refrigerator manualWebPhospho/Total ERK1/2 Whole Cell Lysate Kit Click image to enlarge The Phospho/Total … cydy short interestWebMILLIPLEX MAP Total/Phospho Erk - Cell Signaling Multiplex Assay Intracellular Bead … cye22tshss ge refrigeratorWebOct 15, 2004 · It is for these reasons that phospho-ERK or other endogenous … cye22tshss water filter