WebDialysis is effective for the removal of detergents that have very high CMCs and/or small aggregation numbers, such the N-octyl glucosides. Detergents with low CMCs and large … WebJan 1, 2001 · The action of non-detergent sulphobetaines (NDSBs) as new mild agents for protein purification is described. The solubilization effects of non-detergent sulphobetaines are shown in different examples; all obtained under non-denaturing conditions: (1) microsomal proteins extraction; (2) recovery after dialysis of nuclear proteins; (3) …
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WebFeb 1, 2014 · The most commonly used detergent removal methods include size-based exclusion (dialysis and gel filtration), hydrophobic adsorption, and ion-exchange chromatography (10). The choice of an appropriate method is dictated by inherent properties of the detergents used including hydrophobicity, CMC, micelle size, and charge. WebSep 18, 1980 · Efficiency of detergent removal during the course of several different procedures for membrane protein reconstitution was examined. Reconstitution methods … black as night black as pitch
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WebMar 31, 2024 · These include precipitation (TCA and/or cold acetone), spin columns designed for detergent removal followed by elution of protein, dialysis (e.g. in a Slide-A … WebDetergent removal Detergent removal has traditionally utilized a variety of methods including dialysis, ion exchange chromatography, sucrose gradients, or acid or acetone precipitation. However, all these methods can be labor- and/or time-intensive, or detergent-specific. The proprietary Thermo Scientific™ Detergent Removal resins enable the ... Webmay be removed by dialysis or gel filtration even when the CMC has been exceeded. Table 1 indicates the detergent concentrations that can be removed by dialysis or desalting. … black as night bande annonce vf