WebNov 3, 2015 · Pharmacology and Toxicology graduate with excellent communication, problem solving and organizational skills with a high level of attention to detail, proven ability to work as part of a cross ... WebHarvesting the brain. If the mouse is >2 weeks old, perfuse the mouse with 10 mL 1X PBS through the left ventricle prior to brain harvest. Beginning from the brain stem, cut upward along the sagittal suture as to not …

Measuring Gene Expression from Mammalian Brain Tissue

WebDuring my PhD period I acquired a strong expertise in cellular and molecular biology. In particular, I became an expert in isolation of primary glial cells from rat brain cortex, transfection/infection of primary cells with miRNAs/plasmids and lentivirus, RNA/DNA extraction, RT-PCR, Real time PCR (SYBR green and TaqMan), bioinformatic (big data … WebJul 10, 2024 · Disconnected interacting protein 2 homolog A (DIP2A) is highly expressed in nervous system and respiratory system of developing embryos. However, genes regulated by Dip2a in developing brain and lung have not been systematically studied. Transcriptome of brain and lung in embryonic 19.5 day (E19.5) were compared between wild type and … travel to guyana from uk

Davide Marangon - Research Associate - LinkedIn

WebOur goal is to quantify the diversity of cell types in the adult mouse brain using large-scale single-cell transcriptomics. Towards that goal, we have generated a dataset that includes … WebThe extracted nucleic acid contains unintended acid (ex: when extracting DNA, RNA is also extracted). RA Total RNA Extraction from Brain of Mouse Protocol 1 (15-30 mg) … WebI have used Qiagen kit for RNA extraction from 100 mg normal brain tissue ( human). I am getting 1600 (approx) ng/ul. It was eluted in 25ul nuclease free water. ... (DEG) in mice … travel to iran from uk