WebMar 14, 2007 · K9JA, an antibody for total Tau is localized only in axons, whereas MAP2 visualized by MAP2 clone HM2 antibody (Sigma) only localizes in dendrites. B, Expression of CFP-tau in hippocampal neurons leads to missorting into dendrites. CFP-tau appears in the soma and dendrites and colocalizes with MAP2 (HM2 antibody). WebInterestingly, P2Y12R was found to interact with toxic Tau species as precipitated by pan Tau-K9JA antibody. The microglial P2Y12R interacted more with extracellular Tau oligomers as compared to aggregates, which may signify the binding reactivity of oligomers to P2Y12R (Figure 1H, I). In this scenario, it has been observed for the first time ...
Phospho-Tau (Ser396) Polyclonal Antibody - Thermo Fisher Scientific
WebSep 1, 2010 · The following antibodies and dilutions were used: Tau:K9JA, polyclonal (A-0024, DAKO; 1:5000–1:20,000); 12E8 (phosphoTau pS262 or pS356, monoclonal; gift from P. Seubert, Elan Pharma; 1:1000); anti-Tau [pS214] (polyclonal, Biosource; 1:500); PHF1 (pS396+pS404, monoclonal; gift from P. Davies, Albert Einstein College, Bronx, NY; … WebAug 21, 2024 · (C) Cleaved PARP, phosphorylated pathologic tau (AT8, PHF1), total tau (K9JA) and tubulin (loading control) were immunoblotted from NDC iPSC-derived neuronal cell lysates treated with DMF, TM or TM+PERKi for 12 h. Representative blots are shown, and protein levels were quantified by densitometry and shown relative to DMF-treated … discovery magazine for kids
Effect of Melatonin on Tau aggregation and Tau-mediated cell …
WebSep 8, 2024 · For quantification of Tau levels, the Western blots were probed with pan-Tau antibody K9JA (A-0024, DAKO, Glostrup, Denmark) and analyzed by densitometry. Cytotoxicity Assays. Cytotoxicity was assessed by a LIVE-DEAD assay kit (Molecular Probes, Eugene, OR). For the LIVE-DEAD assay, N2a cells seeded on the coverslips … WebMay 5, 2016 · The axonal-enriched microtubule-associated protein tau is a key mediator of neurotoxicity in Alzheimer's disease and other tauopathies. Using a well characterized in … WebRNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation. View PDF In Acta Neuropathologica … discovery magic set