Tryp le
WebHeadquarters 5791 Van Allen Way Carlsbad, CA 92008 USA Phone +1.760.603.7200 Toll Free in the USA 800.955.6288 www.lifetechnologies.com WebJun 4, 2024 · Cells were cultured for 3 days in the medium specific for each cell type. After 3 days, cells were dissociated from the culture plate using the Tryp-LE reagent, counted with hemocytometer and centrifuged at 400 x g for 5 minutes to pellet them. Supernatant was discarded and cells were re-suspended in 1 ml of WIF water (Millipore/Sigma).
Tryp le
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WebA recombinant enzyme replacing animal trypsin for the dissociation of adherent mammalian cells from plastic. TrypLE™ Express is based on the same enzyme found in TrypLE™ WebMay 24, 2024 · Tryp LE ™ Express is an animal-free recombinant serine protease with trypsin-like activity and can be used to dissociate cells cultured both in serum-free and in serum-supplemented media.
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WebThank you, Victor. I have tried Tryp-LE as well as trypsin. It still occurs. It is primarily transfected cells that round up, no matter how little transfection reagent (or which) and dna I use. WebTrypLE™ Express 是一种非动物源性重组酶，适用于解离各种贴壁哺乳动物细胞，包括 CHO、HEK 293、A529、原代人角质化细胞和胚胎干细胞。. TrypLE™ Express 裂解赖氨酸和精氨酸 C-末端上的肽键，可直接替代胰蛋 …
WebTrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human …
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